Cloned (Comment) | Organism |
---|---|
gene cloning and sequence analysis of the IOR gene, 2 genes iorA and iorB, encoding alpha and beta subunits are tandemly arranged, KOD1-IOR overproduced in anaerobically incubated Escherichia coli BL21(DE3) | Thermococcus kodakarensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24000 | - |
2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
71000 | - |
2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
192000 | - |
gel filtration | Thermococcus kodakarensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin | Pyrococcus furiosus | - |
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin | Thermococcus kodakarensis | - |
S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrococcus furiosus | - |
- |
- |
Thermococcus kodakarensis | - |
KOD1 | - |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
(indol-3-yl)pyruvate + CoA + oxidized ferredoxin | - |
Thermococcus kodakarensis | S-2-(indol-3-yl)acetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
indolepyruvate + CoA + oxidized methylviologen | - |
Pyrococcus furiosus | indoleacetyl-CoA + CO2 + reduced methylviologen | - |
? | |
indolepyruvate + CoA + oxidized methylviologen | - |
Thermococcus kodakarensis | indoleacetyl-CoA + CO2 + reduced methylviologen | - |
? | |
p-hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | p-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
p-hydroxyphenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus kodakarensis | p-hydroxyphenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
phenylpyruvate + CoA + oxidized ferredoxin | - |
Pyrococcus furiosus | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r | |
phenylpyruvate + CoA + oxidized ferredoxin | - |
Thermococcus kodakarensis | phenylacetyl-CoA + CO2 + reduced ferredoxin | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Thermococcus kodakarensis |
tetramer | 2 * 71000 + 2 * 24000, alpha2,beta2, SDS-PAGE | Pyrococcus furiosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Thermococcus kodakarensis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 80 | - |
Thermococcus kodakarensis |